Identification of YhdA as a regulator of the Escherichia coli carbon storage regulation system

FEMS Microbiol Lett. 2006 Nov;264(2):232-7. doi: 10.1111/j.1574-6968.2006.00457.x.


The Escherichia coli BarA-UvrY two-component system, which controls adaptation via the CsrB and CsrC sRNAs, is induced at the entry of the stationary phase by an unknown stimulus. Using a csrB-lacZ fusion, we demonstrated that the factors RelA, SpoT and LuxS, previously suggested to act on orthologues of this system, have no role in BarA-UvrY induction. However, using a transposon screen, we identified the hypothetical protein YhdA as a new regulator of CsrB and CsrC expression. The YhdA protein is predicted to be membrane-bound and to harbor GGDEF and EAL domains, which, however, are highly divergent from the consensus motifs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon / metabolism*
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism*
  • Escherichia coli / physiology
  • Escherichia coli Proteins / isolation & purification*
  • Escherichia coli Proteins / physiology
  • Gene Expression Regulation, Bacterial*
  • Genes, Regulator
  • Membrane Proteins / isolation & purification*
  • Membrane Proteins / physiology


  • CsrD protein, E coli
  • Escherichia coli Proteins
  • Membrane Proteins
  • Carbon