Neural differentiation potential of peripheral blood- and bone-marrow-derived precursor cells

Brain Res. 2006 Dec 6;1123(1):27-33. doi: 10.1016/j.brainres.2006.09.044. Epub 2006 Oct 24.


Transplantation of mesenchymal stem cells (MSCs) prepared from adult bone marrow (BMSCs) has been reported to ameliorate functional deficits in several CNS diseases in experimental animal models. Bone marrow was enriched in MSCs by selecting for plastic-adherent cells that were grown to confluency in appropriate culture conditions as flattened fibroblast-like cells. Despite the fact that the stem/precursor cells in peripheral blood are widely used for reconstruction in the hematopoietic system, it is not fully understood whether peripheral blood-derived plastic-adherent precursor/stem cells (PMSCs) can differentiate into a neural lineage. To compare the potential of PMSCs and BMSCs for neural differentiation in vitro, BMSCs and PMSCs were prepared from the adult rat and expanded in culture. Although the growth rate of PMSCs was less than BMSCs, immunocytochemical and RT-PCR analyses indicated that both MSC types were successfully induced to nestin-positive neurospheres in the presence of EGF and bFGF. After withdrawal of the mitogens, these cells could differentiate into neurofilament-positive neurons or GFAP-positive glia. Thus, our findings suggest the potential use of PMSCs for a cell therapy in CNS diseases.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 5'-Nucleotidase / metabolism
  • Adult Stem Cells / cytology*
  • Adult Stem Cells / metabolism
  • Analysis of Variance
  • Animals
  • Blood Cells / cytology
  • Blood Cells / metabolism
  • Bone Marrow Cells / cytology
  • Cell Adhesion
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Epidermal Growth Factor / physiology
  • Female
  • Fibroblast Growth Factor 2 / physiology
  • Fibroblasts / cytology*
  • Fibroblasts / metabolism
  • Intermediate Filament Proteins / metabolism
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism
  • Nerve Tissue Proteins / metabolism
  • Nestin
  • Neuroglia / cytology*
  • Neuroglia / metabolism
  • Neurons / cytology*
  • Neurons / metabolism
  • Rats
  • Rats, Sprague-Dawley


  • Intermediate Filament Proteins
  • Nerve Tissue Proteins
  • Nes protein, rat
  • Nestin
  • Fibroblast Growth Factor 2
  • Epidermal Growth Factor
  • 5'-Nucleotidase