Study on inactivation kinetics of hepatitis A virus and enteroviruses with peracetic acid and chlorine. New ICC/PCR method to assess disinfection effectiveness

J Prev Med Hyg. 2006 Jun;47(2):56-63.


The virucidal activity of chlorine-compounds was studied using hepatitis A virus (HAV) and Poliovirus 2 and comparing the disinfectant efficiency of peracetic acid. HAV presented a higher resistance to HClO than Poliovirus did. With ClO2 the inactivation times of HAV were markedly shorter. A comparison between these data and those resulting from the kinetics with peracetic acid (PA) showed that PA is less effective than chlorine. As a preliminary to future research, the PCR-test integrated with cell-cultures was experimentally introduced for a quick evaluation of the HAV-infectiveness, with the aim of possible application in the field of disinfection and of viruses-isolation from environmental and food samples.

MeSH terms

  • Cell Culture Techniques
  • Chlorine Compounds / pharmacokinetics
  • Chlorine Compounds / pharmacology
  • Disinfectants / pharmacology*
  • Dose-Response Relationship, Drug
  • Drug Resistance, Viral
  • Enterovirus B, Human / drug effects
  • Hepatitis A virus / drug effects*
  • Hydrogen-Ion Concentration
  • Hypochlorous Acid / pharmacokinetics
  • Hypochlorous Acid / pharmacology
  • Linear Models
  • Logistic Models
  • Oxides / pharmacokinetics
  • Oxides / pharmacology
  • Peracetic Acid / pharmacology
  • Poliovirus / drug effects*
  • Polymerase Chain Reaction
  • Virus Inactivation / drug effects*


  • Chlorine Compounds
  • Disinfectants
  • Oxides
  • Hypochlorous Acid
  • chlorine dioxide
  • Peracetic Acid