qPrimerDepot: a primer database for quantitative real time PCR

Nucleic Acids Res. 2007 Jan;35(Database issue):D805-9. doi: 10.1093/nar/gkl767. Epub 2006 Oct 26.


Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • DNA Primers*
  • Databases, Nucleic Acid*
  • Gene Expression
  • Humans
  • Internet
  • Mice
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • User-Computer Interface


  • DNA Primers