A growing perception in endodontic circles is that root canal therapy requires one treatment visit only. One-visit endodontics offers many potential advantages. It is less time-consuming, resulting in less cost for the patient, potentially more profit for the dentist, less painful and less traumatic than multi-visit treatment. Conversely, if the pulp is necrotic and/or associated with periradicular disease, the root canal system is infected. In these cases, the root canal system should ideally be cleaned, an intracanal medication placed, and the canal filled at a second visit. In this paper we aim to review several aspects of one-visit versus multi-visit endodontic treatment and future perspectives in this field.