Periostin promotes atrioventricular mesenchyme matrix invasion and remodeling mediated by integrin signaling through Rho/PI 3-kinase

Dev Biol. 2007 Feb 1;302(1):256-66. doi: 10.1016/j.ydbio.2006.09.048. Epub 2006 Oct 4.

Abstract

Recent evidence suggests that extracellular matrix components may play a signaling role in embryonic valve development. We have previously identified the spatiotemporal expression patterns of periostin in developing valves, but its function during this process is largely unknown. To evaluate the functional role periostin plays during valvulogenesis, two separate three-dimensional culture assay systems, which model chick atrioventricular cushion development, were employed. These assays demonstrated that cushion mesenchymal cells adhered and spread on purified periostin in a dose-responsive manner, similar to collagen I and fibronectin via alpha(v)beta(3) and beta(1) integrin pairs. Periostin overexpression resulted in enhanced mesenchyme invasion through 3D collagen gels and increased matrix compaction. This invasion was dependent on alpha(v)beta(3) more than beta(1) integrin signaling, and was mediated differentially by Rho kinase and PI 3-kinase. Both matrix invasion and compaction were associated with a colocalization of periostin and beta(1) integrin expression to migratory cell phenotype in both surface and deep cells. The Rho/PI 3-kinase pathway also differentially mediated matrix compaction. Both Rho and PI 3-kinase were involved in normal cushion mesenchyme matrix compaction, but only PI 3-kinase was required for the enhanced matrix compaction due to periostin. Taken together, these results highlight periostin as a mediator of matrix remodeling by cushion mesenchyme towards a mature valve structure.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenoviridae / genetics
  • Animals
  • Cell Adhesion
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism*
  • Cell Adhesion Molecules / physiology
  • Cell Line
  • Chick Embryo
  • Collagen / metabolism
  • Extracellular Matrix / metabolism
  • Heart Valves / embryology
  • Humans
  • In Vitro Techniques
  • Integrins / metabolism*
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Mesoderm / cytology
  • Mesoderm / metabolism*
  • Mice
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Protein-Serine-Threonine Kinases / metabolism*
  • rho-Associated Kinases

Substances

  • Cell Adhesion Molecules
  • Integrins
  • Intracellular Signaling Peptides and Proteins
  • Postn protein, mouse
  • Collagen
  • Phosphatidylinositol 3-Kinases
  • Protein-Serine-Threonine Kinases
  • rho-Associated Kinases