The NSm proteins of Rift Valley fever virus are dispensable for maturation, replication and infection

Virology. 2007 Mar 15;359(2):459-65. doi: 10.1016/j.virol.2006.09.035. Epub 2006 Oct 30.


Rift Valley fever (RVF) virus belongs to the Bunyaviridae family of segmented negative-strand RNA viruses and causes mosquito-borne disease in sub-Saharan Africa. We report the development of a T7 RNA polymerase-driven plasmid-based genetic system for the virulent Egyptian isolate, ZH501. We have used this system to rescue a virus that has a 387 nucleotide deletion on the genomic M segment that eliminates the coding region for two non-structural proteins known as NSm. This virus, DeltaNSm rZH501, is indistinguishable from the parental ZH501 strain with respect to expression of structural proteins and growth in cultured mammalian cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chlorocebus aethiops
  • DNA, Complementary
  • Gene Deletion
  • Molecular Sequence Data
  • Mutation
  • Rift Valley fever virus / genetics
  • Rift Valley fever virus / pathogenicity
  • Rift Valley fever virus / physiology*
  • Vero Cells
  • Viral Proteins / metabolism*
  • Virus Assembly / physiology
  • Virus Replication / physiology*


  • DNA, Complementary
  • Viral Proteins