Abstract
Modulation of cell surface molecules involved in immune recognition and cellular interactions (class I major histocompatibility complex or MHC-I, B7.1 or CD80, integrin alpha4 or CD49d, tetraspanins CD9, CD81) was examined in modified B16 melanoma cells displaying either inhibited IGF-I expression or transfected OVA encoding gene. It was shown that inhibiting IGF-I expression or inserting OVA encoding gene did not lead to modification relevant to the presence of MHC-I or B7.1. However downregulation of tetraspanin CD9 was observed in modified IGF-I but not in OVA encoding gene inserted melanoma cells. Expression of tetraspanin CD81 and integrin alpha4/CD49d remained unchanged. Inoculated into syngeneic recipients, the modified melanoma cells exhibited significant delayed outgrowth with a reduction in the percentage of lethal tumors observed essentially in hosts injected with inhibited IGF-I expression cells.
MeSH terms
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Animals
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Anti-Bacterial Agents / pharmacology
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Antigens, CD / genetics
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Antigens, CD / metabolism
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Antigens, Surface / genetics
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Antigens, Surface / metabolism*
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B7-1 Antigen / genetics
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B7-1 Antigen / metabolism
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Cell Line, Tumor
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Cell Survival / drug effects
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DNA, Antisense / genetics
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Down-Regulation / drug effects
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Electroporation / methods
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Female
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Flow Cytometry
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Gene Expression / drug effects
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Histocompatibility Antigens Class I / genetics
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Histocompatibility Antigens Class I / metabolism
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Hygromycin B / pharmacology
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Immunohistochemistry
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Insulin-Like Growth Factor I / genetics
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Insulin-Like Growth Factor I / metabolism
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Integrin alpha4 / genetics
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Integrin alpha4 / metabolism
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Melanoma, Experimental / genetics
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Melanoma, Experimental / metabolism*
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Melanoma, Experimental / pathology
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred C57BL
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Ovalbumin / genetics
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Ovalbumin / metabolism
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Tetraspanin 28
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Tetraspanin 29
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Transfection / methods
Substances
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Anti-Bacterial Agents
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Antigens, CD
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Antigens, Surface
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B7-1 Antigen
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Cd81 protein, mouse
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Cd9 protein, mouse
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DNA, Antisense
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Histocompatibility Antigens Class I
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Membrane Glycoproteins
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Tetraspanin 28
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Tetraspanin 29
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Integrin alpha4
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Hygromycin B
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Insulin-Like Growth Factor I
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Ovalbumin