Abstract
Ethanol extract of the aerial portion of Chelidonium majus L. inhibited acetylcholinesterase (AChE) activity without a significant inhibition of butyrylcholinesterase (BuChE). Using mass spectrometry and NMR studies, three active constituents were isolated and identified: 8-hydroxydihydrochelerythrine (1), 8-hydroxydihydrosanguinarine (2), and berberine (3). Compounds 1-3 showed potent inhibitory activity against AChE, with IC50 (microM) values of 0.61-1.85. Compound 1 exhibited competitive and selective inhibition for AChE.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylcholinesterase / metabolism
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Berberine / chemistry
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Berberine / isolation & purification
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Berberine / pharmacology
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Butyrylcholinesterase / metabolism
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Chelidonium / chemistry*
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Chloroform / chemistry
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Cholinesterase Inhibitors / chemistry
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Cholinesterase Inhibitors / isolation & purification
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Cholinesterase Inhibitors / pharmacology*
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Chromatography / methods
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Dose-Response Relationship, Drug
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Ethanol / chemistry
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Herbal Medicine
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Heterocyclic Compounds, 4 or More Rings / chemistry
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Heterocyclic Compounds, 4 or More Rings / isolation & purification
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Heterocyclic Compounds, 4 or More Rings / pharmacology*
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Methanol / chemistry
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular / methods
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Plant Components, Aerial / chemistry
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Polycyclic Compounds / chemistry
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Polycyclic Compounds / isolation & purification
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Polycyclic Compounds / pharmacology
Substances
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8-hydroxydihydrochelerythrine
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8-hydroxydihydrosanguinarine
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Cholinesterase Inhibitors
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Heterocyclic Compounds, 4 or More Rings
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Plant Extracts
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Polycyclic Compounds
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Berberine
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Ethanol
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Chloroform
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Acetylcholinesterase
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Butyrylcholinesterase
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Methanol