Enhanced fracture and soft-tissue healing by means of anabolic dietary supplementation

J Bone Joint Surg Am. 2006 Nov;88(11):2386-94. doi: 10.2106/JBJS.F.00507.


Background: Malnutrition is common in hospitalized injured patients. It contributes to delayed fracture-healing and increased morbidity. However, relatively little attention has been directed toward nutritional strategies for augmenting musculoskeletal recovery after a fracture. This animal study was designed to examine the effects of dietary protein intake and the role of conditionally essential amino acids in muscle and bone-healing after a fracture.

Methods: One hundred adult male rats were used. Ten rats served as controls and received a 15% protein diet throughout the study. The remaining ninety rats received a 6% protein diet for five weeks to induce protein malnutrition. The rats underwent intramedullary nailing and closed midshaft fracture of one femur. After the fracture, they were separated into three isocaloric dietary groups. Group P6 received a diet with 6% protein; Group P15, a diet with 15% protein; and group P30, a diet with 30% protein with conditionally essential amino acids. At two, four, and six weeks after surgery, ten animals from each group were killed and the femora were evaluated with dual x-ray absorptiometry, histomorphometric assessment of callus, and torsional testing. The quadriceps muscles were analyzed for total mass, total protein content, and for mRNA expression of insulin-like growth factor-1 (IGF-1), IGF-2, IGF receptors, actin, myosin, and vascular endothelial growth factor (VEGF).

Results: The P30 group demonstrated elevations in albumin, body mass, muscle mass, total protein content of muscle, and bone mineral density in the fracture callus compared with the P6 diet group at six weeks (p < 0.05). Molecular analysis of muscle revealed that IGF-1, IGF-2, IGF receptors, myosin, actin, and VEGF gene expression were significantly (p < 0.001) higher in the P6 group compared with the P30 group. Biomechanical testing of the femora, however, showed no significant differences.

Conclusions: Dietary supplementation with conditionally essential amino acids in malnourished animals had anabolic effects on bone mineralization, body mass, and muscle mass.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorptiometry, Photon
  • Amino Acids, Essential / administration & dosage*
  • Anabolic Agents / administration & dosage*
  • Animals
  • Bony Callus / anatomy & histology
  • Dietary Proteins / administration & dosage
  • Dietary Supplements*
  • Fracture Healing / drug effects*
  • Male
  • Muscle Proteins / analysis
  • Muscle, Skeletal / anatomy & histology
  • Organ Size
  • Protein-Energy Malnutrition / metabolism
  • Rats
  • Wound Healing / drug effects*


  • Amino Acids, Essential
  • Anabolic Agents
  • Dietary Proteins
  • Muscle Proteins