A Rapid Liquid Chromatography-Tandem Mass Spectrometry Method for Quantification of a Biologically Active Molecule Camboginol in the Extract of Garcinia Cambogia

Biomed Chromatogr. 2007 Jan;21(1):55-66. doi: 10.1002/bmc.719.


A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of camboginol in the extract of fruit rinds of Garcinia cambogia has been developed. Separation was achieved isocratically on an RP C(18) column using a solvent system consisting of a mixture of acetonitrile-water (9:1) and methanol-acetic acid (99.5:0.5) in the ratio of 30:70 as mobile phase at a flow rate of 0.4 mL/min. A multiple reaction monitoring (MRM) method was developed for quantification of camboginol in the fruit rinds extract of G. cambogia using MRM transitions of m/z 601.4 --> m/z 176.7 and m/z 601.4 --> m/z 448.9, respectively. The calibration curve based on peak area against concentration was linear up to 50 ng/mL with a detection limit of 0.5 ng/mL. The method showed satisfactory reproducibility with a coefficient of variation of less than 6%. The method was successfully applied for quantification of camboginol in different Garcinia extracts.

MeSH terms

  • Calibration
  • Chromatography, Liquid
  • Garcinia cambogia / chemistry*
  • Molecular Structure
  • Plant Bark / chemistry
  • Reference Standards
  • Seeds / chemistry
  • Tandem Mass Spectrometry
  • Terpenes / analysis*
  • Time Factors


  • Terpenes
  • garcinol