Analysis of oxidative signalling induced by ozone in Arabidopsis thaliana

Plant Cell Environ. 2006 Jul;29(7):1357-71. doi: 10.1111/j.1365-3040.2006.01516.x.


We are using acute ozone as an elicitor of endogenous reactive oxygen species (ROS) to understand oxidative signalling in Arabidopsis. Temporal patterns of ROS following a 6 h exposure to 300 nL L(-1) of ozone in ozone-sensitive Wassilewskija (Ws-0) ecotype showed a biphasic ROS burst with a smaller peak at 4 h and a larger peak at 16 h. This was accompanied by a nitric oxide (NO) burst that peaked at 9 h. An analysis of antioxidant levels showed that both ascorbate (AsA) and glutathione (GSH) were at their lowest levels, when ROS levels were high in ozone-stressed plants. Whole genome expression profiling analysis at 1, 4, 8, 12 and 24 h after initiation of ozone treatment identified 371 differentially expressed genes. Early induction of proteolysis and hormone-responsive genes indicated that an oxidative cell death pathway was triggered rapidly. Down-regulation of genes involved in carbon utilization, energy pathways and signalling suggested an inefficient defense response. Comparisons with other large-scale expression profiling studies indicated some overlap between genes induced by ethylene and ozone, and a significant overlap between genes repressed by ozone and methyl jasmonate treatment. Further, analysis of cis elements in the promoters of ozone-responsive genes also supports the view that phytohormones play a significant role in ozone-induced cell death.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / drug effects*
  • Ascorbic Acid / metabolism
  • Cell Death / drug effects
  • Fumigation
  • Gene Expression Regulation, Plant / drug effects
  • Genes, Plant / genetics
  • Glutathione / metabolism
  • Nitric Oxide / metabolism
  • Oxidation-Reduction / drug effects
  • Ozone / pharmacology*
  • Peptide Hydrolases / metabolism
  • Plant Growth Regulators / pharmacology
  • Promoter Regions, Genetic / genetics
  • Protein Binding / drug effects
  • Signal Transduction / drug effects*
  • Transcription Factors / metabolism


  • Plant Growth Regulators
  • Transcription Factors
  • Nitric Oxide
  • Ozone
  • Peptide Hydrolases
  • Glutathione
  • Ascorbic Acid