Plasma creatinine determination in mice and rats: an enzymatic method compares favorably with a high-performance liquid chromatography assay

Kidney Int. 2007 Jan;71(1):74-8. doi: 10.1038/ Epub 2006 Nov 1.


The use of the colorimetric Jaffé method for the measurement of creatinine in mouse and rat plasma has been criticized as prior studies have shown a dramatic overestimation. We compared a colorimetric picric acid, an enzymatic, and a high-performance liquid chromatography (HPLC) method to assess their appropriateness for routine measurements of creatinine in plasma of healthy and diseased mice (n=61) and rats (n=56). For the colorimetric Jaffé method a pronounced overestimation is confirmed. Additionally the method showed interference with hemoglobin already in a very low, non-visible concentration range in rat plasma. The enzymatic measurement demonstrated a hemoglobin interference in mice, only when hemolysis was visible. The comparison between HPLC and the enzymatic measurement gave a good agreement between both methods in both species. Therefore the enzymatic method fulfills the requirements for a routine screening test for plasma creatinine in healthy as well as diseased mice and rats Kiover a broad concentration range.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases
  • Animals
  • Blood Chemical Analysis / methods*
  • Chromatography, High Pressure Liquid
  • Colorimetry
  • Creatinine / blood*
  • Hemolysis
  • Mice
  • Mice, Mutant Strains
  • Picrates
  • Polycystic Kidney Diseases / blood
  • Polycystic Kidney Diseases / genetics
  • Rats
  • Rats, Mutant Strains
  • Rats, Sprague-Dawley
  • Ureohydrolases


  • Picrates
  • picric acid
  • Creatinine
  • Amidohydrolases
  • creatininase
  • Ureohydrolases
  • creatinase