Human body fluid proteome analysis

Proteomics. 2006 Dec;6(23):6326-53. doi: 10.1002/pmic.200600284.


The focus of this article is to review the recent advances in proteome analysis of human body fluids, including plasma/serum, urine, cerebrospinal fluid, saliva, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate fluid, tear fluid, and amniotic fluid, as well as its applications to human disease biomarker discovery. We aim to summarize the proteomics technologies currently used for global identification and quantification of body fluid proteins, and elaborate the putative biomarkers discovered for a variety of human diseases through human body fluid proteome (HBFP) analysis. Some critical concerns and perspectives in this emerging field are also discussed. With the advances made in proteomics technologies, the impact of HBFP analysis in the search for clinically relevant disease biomarkers would be realized in the future.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Acute-Phase Proteins / chemistry
  • Amniotic Fluid / chemistry
  • Biomarkers / analysis
  • Blood Proteins / chemistry
  • Body Fluids / chemistry*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Cerebrospinal Fluid Proteins / chemistry
  • Female
  • Gene Expression Profiling / methods
  • Glycoproteins / chemistry
  • Humans
  • Lipoproteins / chemistry
  • Nipples / chemistry
  • Pregnancy
  • Protein Array Analysis / methods
  • Proteinuria / urine
  • Proteomics*
  • Salivary Proteins and Peptides / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Synovial Fluid / chemistry
  • Tears / chemistry


  • Acute-Phase Proteins
  • Biomarkers
  • Blood Proteins
  • Cerebrospinal Fluid Proteins
  • Glycoproteins
  • Lipoproteins
  • Salivary Proteins and Peptides