Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei in high fructose fed rats

Nutrition. 2007 Jan;23(1):62-8. doi: 10.1016/j.nut.2006.09.002. Epub 2006 Nov 2.


Objective: We investigated the effect of low-fat (2.5%) dahi containing probiotic Lactobacillus acidophilus and Lactobacillus casei on progression of high fructose-induced type 2 diabetes in rats.

Methods: Diabetes was induced in male albino Wistar rats by feeding 21% fructose in water. The body weight, food and water intakes, fasting blood glucose, glycosylated hemoglobin, oral glucose tolerance test, plasma insulin, liver glycogen content, and blood lipid profile were recorded. The oxidative status in terms of thiobarbituric acid-reactive substances and reduced glutathione contents in liver and pancreatic tissues were also measured.

Results: Values for blood glucose, glycosylated hemoglobin, glucose intolerance, plasma insulin, liver glycogen, plasma total cholesterol, triacylglycerol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, and blood free fatty acids were increased significantly after 8 wk of high fructose feeding; however, the dahi-supplemented diet restricted the elevation of these parameters in comparison with the high fructose-fed control group. In contrast, high-density lipoprotein cholesterol decreased slightly and was retained in the dahi-fed group. The dahi-fed group also exhibited lower values of thiobarbituric acid-reactive substances and higher values of reduced glutathione in liver and pancreatic tissues compared with the high fructose-fed control group.

Conclusion: The probiotic dahi-supplemented diet significantly delayed the onset of glucose intolerance, hyperglycemia, hyperinsulinemia, dyslipidemia, and oxidative stress in high fructose-induced diabetic rats, indicating a lower risk of diabetes and its complications.

MeSH terms

  • Animals
  • Area Under Curve
  • Blood Glucose / analysis
  • Blood Glucose / metabolism
  • Diabetes Mellitus, Type 2 / chemically induced
  • Diabetes Mellitus, Type 2 / prevention & control*
  • Fructose / administration & dosage*
  • Glucose Tolerance Test
  • Glutathione / metabolism
  • Glycated Hemoglobin A / analysis
  • Glycogen / metabolism
  • Insulin / blood
  • Lactobacillus acidophilus / growth & development
  • Lactobacillus acidophilus / physiology*
  • Lactobacillus casei / growth & development
  • Lactobacillus casei / physiology*
  • Lipid Metabolism / drug effects
  • Lipid Metabolism / physiology
  • Male
  • Oxidative Stress / drug effects
  • Probiotics*
  • Rats
  • Rats, Wistar
  • Risk Factors
  • Thiobarbituric Acid Reactive Substances / analysis
  • Yogurt / microbiology*


  • Blood Glucose
  • Glycated Hemoglobin A
  • Insulin
  • Thiobarbituric Acid Reactive Substances
  • Fructose
  • Glycogen
  • Glutathione