Differential Regulation of Germline mRNAs in Soma and Germ Cells by Zebrafish miR-430

Curr Biol. 2006 Nov 7;16(21):2135-42. doi: 10.1016/j.cub.2006.08.086.

Abstract

Early in development, primordial germ cells (PGCs) are set aside from somatic cells and acquire a unique gene-expression program . The mechanisms underlying germline-specific gene expression are largely unknown. Nanos expression is required during germline development and is posttranscriptionally restricted to PGCs . Here we report that the microRNA miR-430 targets the 3' untranslated region (UTR) of nanos1 during zebrafish embryogenesis. A miR-430 target site within the nanos1 3' UTR reduces poly(A) tail length, mRNA stability, and translation. Repression is disrupted in maternal-zygotic dicer mutants (MZdicer), which lack mature miRNAs , and is restored by injection of processed miR-430. Although miR-430 represses other genes equally in germline and soma, specific regions in the nanos1 3' UTR compensate for microRNA-mediated repression in PGCs and allow germline-specific expression. We show that the 3' UTR of an additional PGC-specific gene, TDRD7, is also targeted by miR-430. These results indicate that miR-430 targets the 3' UTRs of germline genes and suggest that differential susceptibility to microRNAs contributes to tissue-specific gene expression.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Base Sequence
  • Gene Expression Regulation, Developmental*
  • Germ Cells / metabolism*
  • MicroRNAs / genetics*
  • Molecular Sequence Data
  • Polyadenylation
  • RNA Interference
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins
  • Ribonuclease III / genetics
  • Zebrafish / embryology*
  • Zebrafish / genetics*
  • Zebrafish Proteins / genetics

Substances

  • 3' Untranslated Regions
  • MicroRNAs
  • RNA, Messenger
  • RNA-Binding Proteins
  • Zebrafish Proteins
  • nanos3 protein, zebrafish
  • Ribonuclease III