A PCR-based method for detection and quantification of small RNAs

Seungil Ro; Chanjae Park; Jingling Jin; Kenton M Sanders; Wei Yan

doi:10.1016/j.bbrc.2006.10.105

A PCR-based method for detection and quantification of small RNAs

Biochem Biophys Res Commun. 2006 Dec 22;351(3):756-63. doi: 10.1016/j.bbrc.2006.10.105. Epub 2006 Oct 30.

Authors

Seungil Ro¹, Chanjae Park, Jingling Jin, Kenton M Sanders, Wei Yan

Affiliation

¹ Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA.

Abstract

Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Gene Expression Profiling / methods*
Mice
MicroRNAs / genetics*
MicroRNAs / metabolism*
Molecular Sequence Data
Organ Specificity
Polymerase Chain Reaction / methods*
Tissue Distribution

Substances

MicroRNAs

Abstract

Publication types

MeSH terms

Substances

Grants and funding