A PCR-based method for detection and quantification of small RNAs

Biochem Biophys Res Commun. 2006 Dec 22;351(3):756-63. doi: 10.1016/j.bbrc.2006.10.105. Epub 2006 Oct 30.


Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Gene Expression Profiling / methods*
  • Mice
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • Molecular Sequence Data
  • Organ Specificity
  • Polymerase Chain Reaction / methods*
  • Tissue Distribution


  • MicroRNAs