Conjugative transfer facilitates stable maintenance of IncP-1 plasmid pKJK5 in Escherichia coli cells colonizing the gastrointestinal tract of the germfree rat

Appl Environ Microbiol. 2007 Jan;73(1):341-3. doi: 10.1128/AEM.01971-06. Epub 2006 Nov 3.


Quantitative determination of IncP-1 plasmid loss from Escherichia coli cells colonizing the gastrointestinal tracts of germfree rats was achieved by flow cytometry. Results show that the plasmid's ability to conjugate counteracts plasmid loss and is thus an important mechanism for the stable maintenance of IncP-1 plasmids within the gastrointestinal environment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Conjugation, Genetic*
  • Escherichia coli / genetics
  • Escherichia coli / growth & development*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Female
  • Flow Cytometry
  • Gastrointestinal Tract / microbiology*
  • Germ-Free Life*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Plasmids / genetics*
  • Rats
  • Rats, Sprague-Dawley


  • Escherichia coli Proteins
  • Green Fluorescent Proteins