Molecular Cloning of a New Class of Cartilage-Specific Matrix, chondromodulin-I, Which Stimulates Growth of Cultured Chondrocytes

Biochem Biophys Res Commun. 1991 Mar 29;175(3):971-7. doi: 10.1016/0006-291x(91)91660-5.

Abstract

Here we report the structure and bioactivity of 25 kDa glycoprotein (chondromodulin-I) as a tissue-specific functional matrix component identified and cloned for the first time. Chondromodulin-I purified from fetal bovine cartilage markedly stimulated DNA synthesis of cultured growth-plate chondrocytes in the presence of basic fibroblast growth factor (FGF). Bovine chondromodulin-I cDNA revealed that the mature protein consists of 121 amino acids with three possible glycosylation sites and is coded as the C-terminal part of a larger precursor. On northern blot analysis, expression of chondromodulin-I mRNA was observed only in cartilage.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cattle
  • Cell Division / drug effects
  • Cells, Cultured
  • Cloning, Molecular
  • DNA Replication / drug effects
  • Female
  • Fetus
  • Gene Library
  • Growth Plate / cytology*
  • Growth Plate / drug effects
  • Growth Plate / physiology
  • Growth Substances / genetics*
  • Growth Substances / isolation & purification
  • Growth Substances / pharmacology
  • Guinea Pigs
  • Intercellular Signaling Peptides and Proteins*
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Oligonucleotide Probes
  • Organ Specificity
  • Polymerase Chain Reaction / methods
  • Pregnancy
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Rabbits
  • Sequence Homology, Nucleic Acid

Substances

  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Oligonucleotide Probes
  • RNA, Messenger
  • RNA

Associated data

  • GENBANK/D00690
  • GENBANK/D00691
  • GENBANK/M62476
  • GENBANK/M62477
  • GENBANK/M62478
  • GENBANK/M62479
  • GENBANK/M62480
  • GENBANK/M62481
  • GENBANK/M62482
  • GENBANK/M65081