Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis

Clin Vaccine Immunol. 2007 Jan;14(1):110-3. doi: 10.1128/CVI.00289-06. Epub 2006 Nov 8.


Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / blood*
  • Antibodies, Bacterial / immunology
  • Antigens, Bacterial* / immunology
  • Burkholderia pseudomallei / immunology*
  • Enzyme-Linked Immunosorbent Assay*
  • Hemagglutination Inhibition Tests
  • Melioidosis / diagnosis*
  • Melioidosis / epidemiology
  • Sensitivity and Specificity
  • Serologic Tests


  • Antibodies, Bacterial
  • Antigens, Bacterial