Shifts of anti-Sm-specific antibodies in patients with systemic lupus erythematosus: analysis by counter-immunoelectrophoresis, immunoblotting and RNA-immunoprecipitation

J Autoimmun. 1991 Feb;4(1):155-64. doi: 10.1016/0896-8411(91)90014-4.


We investigated serial changes in anti-Sm-specific antibodies by counter-immunoelectrophoresis (CIE), immunoblotting (IB) and RNA-immunoprecipitation (RNA-IP) in three patients with SLE. To assess the immuno-regulatory processes underlying anti-Sm antibody production, we compared changes in anti-Sm-specific antibodies with changes in titres of antinuclear and anti-ds DNA antibodies. IB and RNA-IP detected anti-Sm antibodies earlier than did CIE. The induction of the immune responses against the Sm-specific BB' and D polypeptides occurred separately in two of the three patients. Anti-Sm developed in all three cases after a flare-up of disease, whereas anti-ds DNA antibody levels fluctuated in parallel with disease activity. Thus, anti-Sm and anti-ds DNA antibody production seems to be independently regulated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Antinuclear / blood
  • Autoantibodies / blood*
  • Autoantigens*
  • Counterimmunoelectrophoresis
  • DNA / immunology
  • Female
  • Humans
  • Immunoblotting
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • Precipitin Tests
  • RNA
  • Ribonucleoproteins / immunology
  • Ribonucleoproteins, Small Nuclear*
  • snRNP Core Proteins


  • Antibodies, Antinuclear
  • Autoantibodies
  • Autoantigens
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • snRNP Core Proteins
  • RNA
  • DNA