Analysis of interstrain variation in cytomegalovirus glycoprotein B sequences encoding neutralization-related epitopes

J Infect Dis. 1991 Jun;163(6):1229-34. doi: 10.1093/infdis/163.6.1229.


Nucleotide sequences of a part of the envelope glycoprotein B (gB) gene of human cytomegalovirus (CMV), encoding epitopes recognized by virus-neutralizing monoclonal antibodies, were determined for 12 distinct clinical strains of CMV after amplification of suitable templates using the polymerase chain reaction. Sequence analysis of this region (codons 384-717) revealed that the clinical strains and previously sequenced laboratory strains Towne and AD169 belong to one of four variant groups, each with a characteristic nucleotide and peptide sequence. Peptide homology was greater than 99% for strains within a group, and varied from 91% to 98% for strains in different groups. Variation was most frequent between codons 448 and 480. The gB group of a CMV strain could be determined by restriction analysis of a small target sequence amplified from viral genomic DNA, and an additional 28 clinical strains were grouped in this manner. The existence of a limited number of variants of gB among clinical strains facilitates analysis of biologic function and cross-reactivity of immune responses.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cytomegalovirus / genetics*
  • Cytomegalovirus / immunology
  • DNA, Viral / chemistry
  • Epitopes / chemistry
  • Epitopes / genetics
  • Genetic Variation*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / genetics*


  • DNA, Viral
  • Epitopes
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus

Associated data

  • GENBANK/M60923
  • GENBANK/M60924
  • GENBANK/M60925
  • GENBANK/M60926
  • GENBANK/M60927
  • GENBANK/M60928
  • GENBANK/M60929
  • GENBANK/M60930
  • GENBANK/M60931
  • GENBANK/M60932
  • GENBANK/M60933
  • GENBANK/M60934