Structure and localization of the human insulin-like growth factor-binding protein 2 gene

Biochem Biophys Res Commun. 1991 May 15;176(3):1250-5. doi: 10.1016/0006-291x(91)90420-c.

Abstract

Insulin-like growth factor binding proteins (IGFBPs) are extracellular proteins that specifically bind IGF and modulate their effects. The human IGFBP2 gene was studied and shown to be localized to chromosome 2 region q33-q34, by somatic cell hybrid analysis and in situ hybridization. Structural characterization of the gene showed that it consists of four exons with three introns of lengths 27.0, 1.0, and 1.9 kilobase-pairs. Comparison of the encoded protein sequence of each exon in IGFBP1, 2, and 3 reveals the highest amino acid identity, 28%, in exon 1, while the lowest was found in exon 2. However, pairwise sequence comparisons demonstrate 50% identity between the protein sequences encoded by exon 4 in IGFBP1 and 2, while their respective identities with IGFBP3 are only 25 and 30%.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Line
  • Chromosomes, Human, Pair 2*
  • Cloning, Molecular
  • Cosmids
  • Cricetinae
  • DNA / genetics
  • DNA / isolation & purification
  • Exons
  • Female
  • Genes*
  • Genomic Library
  • Humans
  • Hybrid Cells / physiology
  • Insulin-Like Growth Factor Binding Protein 2
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Placenta / metabolism
  • Polymerase Chain Reaction / methods
  • Pregnancy
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Carrier Proteins
  • Insulin-Like Growth Factor Binding Protein 2
  • Oligonucleotide Probes
  • DNA