Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins

Cell. 1991 May 31;65(5):859-73. doi: 10.1016/0092-8674(91)90393-d.


Rolling of leukocytes on vascular endothelial cells, an early event in inflammation, can be reproduced in vitro on artificial lipid bilayers containing purified CD62, a selectin also named PADGEM and GMP-140 that is inducible on endothelial cells. Neutrophils roll on this selectin under flow conditions similar to those found in postcapillary venules. Adhesion of resting or activated neutrophils through the integrins LFA-1 and Mac-1 to ICAM-1 in a lipid bilayer does not occur at physiologic shear stresses; however, static incubation of activated neutrophils allows development of adhesion that is greater than 100-fold more shear resistant than found on CD62. Addition of a chemoattractant to activate LFA-1 and Mac-1 results in the arrest of neutrophils rolling on bilayers containing both CD62 and ICAM-1. Thus, at physiologic shear stress, rolling on a selectin is a prerequisite for activation-induced adhesion strengthening through integrins.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Adhesion Molecules / physiology*
  • Cell Adhesion*
  • Cell Movement
  • Humans
  • In Vitro Techniques
  • Integrins / physiology*
  • Lipid Bilayers
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • P-Selectin
  • Platelet Membrane Glycoproteins / physiology*
  • Stress, Mechanical
  • Tetradecanoylphorbol Acetate / pharmacology


  • Cell Adhesion Molecules
  • Integrins
  • Lipid Bilayers
  • P-Selectin
  • Platelet Membrane Glycoproteins
  • N-Formylmethionine Leucyl-Phenylalanine
  • Tetradecanoylphorbol Acetate