Biocompatibility of electrophoretical deposition of nanostructured hydroxyapatite coating on roughen titanium surface: in vitro evaluation using mesenchymal stem cells

J Biomed Mater Res B Appl Biomater. 2007 Jul;82(1):183-91. doi: 10.1002/jbm.b.30720.


A nano hydroxyapatite (HAp) layer was coated on a roughen titanium surface by means of electrophoretic deposition with an acetic anhydride solvent system. The objectives of this current study are to investigate whether nano-HAp can improve mechanical strength at a lower sintering temperature and biocompatibility. Densification temperature was lowered from usual 1000 to 800 degrees C. The coating interfacial bonding strength, phase purity, microstructure, and biocompatibility were investigated. Degradation of HA phase was not detected in XRD. A porous TiO2 layer acts as a gradient coating layer with an intermediate thermal expansion coefficient between hydroxyapatite and titanium that reduces the thermal stress. From SEM image, the coating does not contain any crack. Mesenchymal stem cell (MSC) is the progenitor cell for various tissues in mature animals, which can improve integration of bone tissue into implant. In this in vitro study, rabbit MSCs culture indicated that the HAp/Ti nanocomposite biomaterial had good biocompatibility and bioactivity. Around materials and on its surface cell grew well with good morphology. Proliferation of the MSCs on the nano-HAp coating was higher than its micron counterpart in XTT assay. These properties show potential for the orthopaedic and dental applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coated Materials, Biocompatible / chemistry
  • Coated Materials, Biocompatible / pharmacology*
  • Durapatite / chemistry
  • Durapatite / pharmacology*
  • Electrophoresis
  • Materials Testing
  • Mesenchymal Stem Cells / drug effects*
  • Nanocomposites / chemistry*
  • Particle Size
  • Porosity
  • Rabbits
  • Stress, Mechanical
  • Surface Properties
  • Titanium / chemistry*
  • X-Ray Diffraction


  • Coated Materials, Biocompatible
  • titanium dioxide
  • Durapatite
  • Titanium