Transcriptional repression of the mouse wee1 gene by TBP-related factor 2

Biochem Biophys Res Commun. 2007 Jan 5;352(1):21-8. doi: 10.1016/j.bbrc.2006.10.175. Epub 2006 Nov 7.


TBP-related factor 2 (TRF2), one of the TBP family proteins, is involved in various cellular functions through its transcription stimulation activity. We previously reported that TRF2 is involved in reduction of wee1 mRNA in genotoxin-treated chicken cells. In this study, we investigated the role of TRF2 in wee1 gene expression. It was found that wee1 mRNA was decreased in hydroxyurea-treated NIH3T3 cells. Mouse wee1 promoter activity was repressed by TRF2 in mouse and chicken cells. Chromatin immunoprecipitation and plasmid immunoprecipitation analyses revealed that TRF2 is recruited to the wee1 promoter in accordance with the transcriptional repression. A mutant TRF2 that lacks TFIIA-binding capacity lost its repressive function. This mutant was less recruited to the wee1 promoter than was the wild-type one, and provided a decline in promoter-recruited TFIIA. Data in this study suggest that transcription repressive activity of TRF2 to wee1 promoter needs association with the promoter and TFIIA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism*
  • Cell Line
  • Chickens
  • Gene Expression / drug effects
  • Hydroxyurea / pharmacology
  • Mice
  • Mutation / genetics
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic / genetics
  • Protein Binding
  • Protein-Tyrosine Kinases / genetics*
  • Protein-Tyrosine Kinases / metabolism*
  • RNA, Messenger / genetics
  • TATA Box Binding Protein-Like Proteins / genetics
  • TATA Box Binding Protein-Like Proteins / metabolism*
  • Transcription, Genetic / genetics*


  • Cell Cycle Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • TATA Box Binding Protein-Like Proteins
  • Protein-Tyrosine Kinases
  • Wee1 protein, mouse
  • Hydroxyurea