High-performance liquid chromatographic determination of bleomycins

J Chromatogr. 1991 Jan 4;536(1-2):343-7. doi: 10.1016/s0021-9673(01)89268-2.


A rapid and specific ion-pair reversed-phase high-performance liquid chromatographic method was developed for the determination of bleomycins. The use of 5-microns particles of less adsorptive reversed-phase packings and sodium perchlorate as ion-pairing reagent permitted a short analysis time and the transferability of the separations on different batches of the reversed-phase materials. The detection sensitivity and precision of the method demonstrated that the system is suitable for routine analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bleomycin / analysis*
  • Chromatography, High Pressure Liquid
  • Spectrophotometry, Ultraviolet


  • Bleomycin