Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells

J Immunol. 1991 Jul 1;147(1):22-8.

Abstract

Murine bone marrow cells expressing the cell surface Ag RB6-8C5 were identified by fluorescence-activated cell-sorting analysis using a rat IgG mAb. The fluorescent intensity of RB6-8C5 was variable on bone marrow cells. This made it possible to separate bone marrow cells into distinct subpopulations, RB6-8C5neg, RB6-8C5lo, and RB6-8C5hi cells. Morphologic analysis of the sorted populations demonstrated that the Ag was expressed on myeloid cells. The expression of RB6-8C5 increases with granulocyte maturation, whereas expression is transient on cells in the monocytic lineage. The RB6-8C5hi sorted cells were enriched for end-stage neutrophils (75%), whereas the RB6-8C5lo sorted cells contained more immature myeloid cells and myelocytes (75%). Lymphocytes and macrophages were less than 5% in any RB6-8C5+ population, whereas the erythroid precursors were RB6-8C5neg. The colony forming unit culture (CFU-C) (greater than 90%) were found in the RB6-8C5neg and RB6-8C5lo populations, and all the CFU-granulocyte, erythroid, megakaryocyte, and macrophage (CFU-GEMM) and burst-forming units-erythroid (BFU-E) were in the RB6-8C5neg population. Granulocyte-macrophage-CSFR (GM-CSFR) and IL-1 alpha R were expressed on RB6-8C5hi bone marrow cells, whereas no receptors could be detected on RB6-8C5neg and RB6-8C5lo cells. The expression of the RB6-8C5 Ag can be induced on RB6-8C5neg cells in liquid culture by IL-3 and granulocyte-macrophage CSF. Thus, RB6-8C5 is a myeloid differentiation Ag whose expression can be regulated by cytokines.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Differentiation, Myelomonocytic / immunology*
  • Antigens, Surface / immunology*
  • Bone Marrow / immunology*
  • Bone Marrow Cells
  • Cell Differentiation
  • Cell Division / drug effects
  • Cell Separation
  • Flow Cytometry
  • Granulocyte Colony-Stimulating Factor / pharmacology
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Hematopoiesis
  • Interleukin-3 / pharmacology
  • Macrophage Colony-Stimulating Factor / pharmacology
  • Mice
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin-1
  • Spleen / cytology

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, Myelomonocytic
  • Antigens, Surface
  • Interleukin-3
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
  • Receptors, Immunologic
  • Receptors, Interleukin-1
  • Granulocyte Colony-Stimulating Factor
  • Macrophage Colony-Stimulating Factor
  • Granulocyte-Macrophage Colony-Stimulating Factor