Charting of type II glucocorticoid receptor-like immunoreactivity in the rat central nervous system

Neuroscience. 1990;39(3):579-604. doi: 10.1016/0306-4522(90)90244-x.


The rat brain and spinal cord have been mapped for Type II glucocorticoid receptor-like immunoreactivity in neurons and glia, using a monoclonal antibody, BUGR2, which recognizes an epitope close to the DNA-binding domain of the rat Type II receptor. The study revealed a widespread distribution of Type II-like immunoreactive neurons and glia, and a heterogeneity of densities and intensities of immunoreactive elements. Our results corresponded to a large extent with previous immunocytochemical mapping using Ig2a, a monoclonal antibody against a different epitope in the variable domain, with some notable differences in the hippocampus, hypothalamus and cerebellum. There was also a good correlation between immunocytochemical mapping and binding studies, [3H]steroid autoradiography and mRNA localization of the Type II receptor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Autoradiography
  • Brain / anatomy & histology*
  • Brain / cytology
  • Epitopes / analysis
  • Male
  • Organ Specificity
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Inbred Strains
  • Receptors, Glucocorticoid / analysis*
  • Receptors, Glucocorticoid / genetics
  • Receptors, Glucocorticoid / immunology
  • Spinal Cord / anatomy & histology*
  • Spinal Cord / cytology
  • Tritium


  • Antibodies, Monoclonal
  • Epitopes
  • RNA, Messenger
  • Receptors, Glucocorticoid
  • Tritium