A peptide with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Russula paludosa

Peptides. 2007 Mar;28(3):560-5. doi: 10.1016/j.peptides.2006.10.004. Epub 2006 Nov 17.


Hot water extracts of 16 species of mushrooms, including both edible and medicinal mushrooms, were screened for human immunodeficiency virus (HIV)-1 reverse transcriptase (RT) inhibitory activity. Extracts of Lactarius camphoratus, Trametes suaveolens, Sparassis crispa, Pleurotus sajor-caju, Pleurotus pulmonarius, and Russula paludosa elicited over 50% inhibition when tested at the concentration of 1 mg/ml. The extract of R. paludosa demonstrated the highest inhibitory activity on HIV-1 RT (97.6%). Fraction SU2, purified from R. paludosa extract by anion exchange chromatography on DEAE-cellulose and gel filtration on Superdex 75, exhibited potent inhibitory activity on HIV-1 RT. At the concentrations of 1 mg/ml, 0.2 mg/ml, and 0.04 mg/ml, the inhibition ratios were 99.2%, 89.3%, and 41.8%, respectively, giving an IC50 of 11 microM. The molecular mass of SU2 was 4.5 kDa and its N-terminal amino acid sequence was determined to be KREHGQHCEF. The peptide was devoid of hemagglutinating, ribonuclease, antifungal, protease, protease inhibitory, and laccase activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / chemistry*
  • Agaricales / genetics
  • Amino Acid Sequence
  • Anti-HIV Agents / isolation & purification*
  • Anti-HIV Agents / pharmacology
  • Fungal Proteins / genetics
  • Fungal Proteins / isolation & purification*
  • Fungal Proteins / pharmacology
  • HIV Reverse Transcriptase / antagonists & inhibitors*
  • Peptides / genetics
  • Peptides / isolation & purification*
  • Peptides / pharmacology
  • Reverse Transcriptase Inhibitors / isolation & purification*
  • Reverse Transcriptase Inhibitors / pharmacology


  • Anti-HIV Agents
  • Fungal Proteins
  • Peptides
  • Reverse Transcriptase Inhibitors
  • HIV Reverse Transcriptase