CHO glycosylation mutants: proteoglycans

Methods Enzymol. 2006;416:205-21. doi: 10.1016/S0076-6879(06)16013-9.

Abstract

Most glycosaminoglycan (GAG)-defective mutants have been isolated and characterized from Chinese hamster ovary (CHO) cells. Wild-type and GAG-defective CHO cells have been used by several hundreds of laboratories to study how altering the GAG structure of proteoglycans affects fundamental properties of cells, such as bacterial/viral infection, signaling, protein degradation, and cell adhesion. This chapter describes methods used to construct and characterize new CHO cell lines with gain-of-function GAG structures. These novel CHO cell lines allow herpes simplex virus (HSV) entry or have anticoagulant properties that are not possessed by wild-type CHO cells. The method used to study GAG biosynthetic mechanisms that control specific GAG sequence assembly is also described.

MeSH terms

  • Animals
  • CHO Cells
  • Cell Line
  • Cricetinae
  • Cricetulus
  • Glycosaminoglycans / biosynthesis*
  • Glycosaminoglycans / genetics*
  • Glycosylation
  • Mutation*
  • Proteoglycans / biosynthesis*
  • Proteoglycans / genetics*

Substances

  • Glycosaminoglycans
  • Proteoglycans