Sensitive and specific method for detecting G protein-coupled receptor mRNAs

Nat Methods. 2007 Jan;4(1):35-7. doi: 10.1038/nmeth977. Epub 2006 Nov 19.


G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction / methods*
  • RNA, Messenger / analysis*
  • RNA, Messenger / genetics*
  • Receptors, G-Protein-Coupled / genetics*
  • Sensitivity and Specificity


  • RNA, Messenger
  • Receptors, G-Protein-Coupled