Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library

Nat Biotechnol. 2006 Dec;24(12):1569-71. doi: 10.1038/nbt1264. Epub 2006 Nov 19.


The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield (0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Flow Cytometry
  • Gene Library*
  • Luminescent Measurements
  • Luminescent Proteins / biosynthesis*
  • Luminescent Proteins / genetics
  • Photobleaching
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / genetics


  • Luminescent Proteins
  • Recombinant Proteins