A promoter switch that can rescue a plant sigma factor mutant

FEBS Lett. 2006 Dec 11;580(28-29):6617-22. doi: 10.1016/j.febslet.2006.11.010. Epub 2006 Nov 16.

Abstract

Chloroplasts sigma factors act in concert with PEP, the bacterial-type plastid RNA polymerase. Using a sigma knockout line from Arabidopsis thaliana, we investigated mutant-specific changes in plastid gene expression at RNA level. One characteristic feature was the appearance of a long transcript that spans the atpB-E operon and extends considerably into the far-upstream region of atpB. This region reveals a cluster of typical promoter elements for NEP, the second (phage-type) plastid RNA polymerase. The NEP promoter cluster can help maintain RNA synthesis in situations where no functional sigma factor is available for PEP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics*
  • Base Sequence
  • DNA, Chloroplast / genetics
  • DNA-Directed RNA Polymerases / genetics
  • Gene Expression Regulation, Plant*
  • Models, Genetic
  • Molecular Sequence Data
  • Molecular Weight
  • Mutant Proteins / metabolism
  • Mutation / genetics*
  • Promoter Regions, Genetic / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sigma Factor / genetics*

Substances

  • Arabidopsis Proteins
  • DNA, Chloroplast
  • Mutant Proteins
  • RNA, Messenger
  • Sigma Factor
  • DNA-Directed RNA Polymerases