Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice

Inflamm Res. 2006 Nov;55(11):457-64. doi: 10.1007/s00011-006-5161-8.


Objective: We investigated the antiinflammatory properties of a derived fraction of tetranortriterpenoids (TNTP) obtained from the seeds of Carapa guianensis Aublet.

Material and methods: Zymosan-induced arthritis and pleurisy in Swiss and C57/Bl6 mice (n = 10 per group). Western blot analysis was performed to analyze nuclear factor-kappaB (NFkappaB) translocation in mice peritoneal macrophages stimulated in vitro with zymosan (500 microg/ml). ELISA was performed to evaluate cytokine levels in knee joints. Values of p </= 0.05 were regarded as significant.

Results: Zymosan intra-articular (i. a.) injection (500microg/ cavity) induced a significant increase in knee joint diameter within 6 h, peaked within 24 h and remained above control values for 20 days. Orally-given (p. o.) TNTP (100-200 mg/ kg) inhibited zymosan-induced increase in knee joint diameter and protein extravazation into synovial cavity within 6 h. TNTP (100-200 mg/kg, p. o.) also inhibited total leukocyte influx into the synovial space and tissue, as well as into the mice pleural cavity, due to neutrophil impairment 6 h after zymosan stimulation. The increase in TNF-alpha, IL-1beta and CXCL8/IL-8 levels that were detected in knee synovial extracts obtained from zymosan-stimulated mice was also inhibited by TNTP (100 mg/kg, p. o.). Moreover, the incubation of mice peritoneal macrophages with TNTP (100 mug/ml) inhibited zymosan (500 microg/ml)-induced NFkappaB translocation into the nucleus 6 h after stimulation.

Conclusion: Taken together, these results indicate that TNTP present an important antiinflammatory effect, inhibiting zymosan-induced arthritis in mice via the impairment of TNF-alpha, IL-1beta and CXCL8/IL-8 generation, as well as NFkappaB signaling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Arthritis, Experimental / chemically induced
  • Arthritis, Experimental / drug therapy*
  • Arthritis, Experimental / metabolism
  • Cytokines / metabolism
  • Dose-Response Relationship, Drug
  • Knee Joint / metabolism
  • Knee Joint / pathology
  • Limonins / pharmacology*
  • Macrophages, Peritoneal
  • Male
  • Meliaceae*
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / drug effects
  • NF-kappa B / metabolism
  • Phytotherapy / methods
  • Plant Preparations / pharmacology*
  • Seeds
  • Synaptotagmin I / metabolism
  • Synovial Membrane / metabolism
  • Synovial Membrane / pathology
  • Zymosan


  • Anti-Inflammatory Agents
  • Cytokines
  • Limonins
  • NF-kappa B
  • Plant Preparations
  • Synaptotagmin I
  • Zymosan