Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway

FEBS Lett. 2006 Dec 11;580(28-29):6635-43. doi: 10.1016/j.febslet.2006.11.014. Epub 2006 Nov 15.

Abstract

Visfatin was originally identified as a growth factor for immature B cells, and recently demonstrated to bind insulin receptor. Visfatin mRNA and protein were detected by RT-PCR and Western blot analysis in cloned bovine mammary epithelial cells, lactating bovine mammary gland and human breast cancer cell line, MCF-7. Immunocytochemical staining localized the visfatin protein in the cytosol and nucleus of both cells. Quantitative-RT-PCR analysis revealed that the expression of the visfatin mRNA was significantly elevated when treated with forskolin (500 microM), isopreterenol (1-10 microM) and dibutyric cyclic AMP (1 mM) for 24 h, and significantly reduced when treated with insulin (5-50 ng/ml) and dexsamethasone (0.5-250 nM) for 24 h. These results indicate that mammary epithelial cells express the visfatin protein and secrete them into the milk.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Flanking Region / genetics
  • Activating Transcription Factor 2 / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cyclic AMP / metabolism*
  • Cyclic AMP Response Element-Binding Protein / genetics
  • Cytokines / chemistry
  • Cytokines / genetics*
  • Cytokines / metabolism*
  • Cytosol / metabolism
  • Dexamethasone / pharmacology
  • Epithelial Cells / cytology*
  • Epithelial Cells / drug effects
  • Female
  • Humans
  • Insulin / pharmacology
  • Lactation / drug effects
  • Lactation / metabolism*
  • Mammary Glands, Animal / cytology*
  • Mammary Glands, Animal / drug effects
  • Milk / chemistry*
  • Molecular Sequence Data
  • Oxytocin / pharmacology
  • Protein Transport / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • Up-Regulation / drug effects

Substances

  • Activating Transcription Factor 2
  • Cyclic AMP Response Element-Binding Protein
  • Cytokines
  • Insulin
  • RNA, Messenger
  • Oxytocin
  • Dexamethasone
  • Cyclic AMP

Associated data

  • RefSeq/XM_878509