Abstract
1Alpha,25-Dihydroxyvitamin D(3) (1,25-(OH)(2)D(3)), the biologically active form of vitamin D(3), not only plays a major role in mammalian calcium and phosphorous homeostasis but also exerts pleiotropic effects on cell proliferation, differentiation and the immune system. Further, vitamin D is believed to play a significant role in the prevention of colon, prostate, and breast cancer and in reducing the risk of autoimmune diseases. To gain insight into the mechanism whereby vitamin D can have such diverse actions, we have employed microarray technology. We studied the effect of a single dose of 1,25-(OH)(2)D(3) on gene expression in the intestine of vitamin D-deficient rats. Within 6 h, 1,25-(OH)(2)D(3) stimulates the expression of several phase I and phase II biotransformation genes. There is also an increased expression of antioxidant genes. These results support the idea that vitamin D is a significant factor in detoxification and protection against environmental toxins.
MeSH terms
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Animals
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Antioxidants / metabolism
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Biotransformation
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Calcitriol / pharmacology*
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Cytochrome P-450 Enzyme System / genetics
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Cytochrome P-450 Enzyme System / metabolism
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Disease Models, Animal
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Duodenum / drug effects*
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Duodenum / enzymology*
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Epoxide Hydrolases / genetics
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Epoxide Hydrolases / metabolism
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Gene Expression Profiling
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Gene Expression Regulation, Enzymologic / drug effects*
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Heme Oxygenase (Decyclizing) / genetics
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Heme Oxygenase (Decyclizing) / metabolism
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Inactivation, Metabolic / genetics*
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Male
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Metabolic Detoxication, Phase I / genetics
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Metabolic Detoxication, Phase II / genetics
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Oligonucleotide Array Sequence Analysis
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Reproducibility of Results
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Reverse Transcriptase Polymerase Chain Reaction
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Steroid Hydroxylases / genetics
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Steroid Hydroxylases / metabolism
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Time Factors
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Transferases / genetics
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Transferases / metabolism
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Vitamin D Deficiency
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Vitamins / pharmacology*
Substances
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Antioxidants
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RNA, Messenger
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Vitamins
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Cytochrome P-450 Enzyme System
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Steroid Hydroxylases
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Heme Oxygenase (Decyclizing)
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Transferases
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Epoxide Hydrolases
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Calcitriol