1,25-Dihydroxyvitamin D3 regulates genes responsible for detoxification in intestine

Toxicol Appl Pharmacol. 2007 Jan 1;218(1):37-44. doi: 10.1016/j.taap.2006.10.005. Epub 2006 Oct 13.


1Alpha,25-Dihydroxyvitamin D(3) (1,25-(OH)(2)D(3)), the biologically active form of vitamin D(3), not only plays a major role in mammalian calcium and phosphorous homeostasis but also exerts pleiotropic effects on cell proliferation, differentiation and the immune system. Further, vitamin D is believed to play a significant role in the prevention of colon, prostate, and breast cancer and in reducing the risk of autoimmune diseases. To gain insight into the mechanism whereby vitamin D can have such diverse actions, we have employed microarray technology. We studied the effect of a single dose of 1,25-(OH)(2)D(3) on gene expression in the intestine of vitamin D-deficient rats. Within 6 h, 1,25-(OH)(2)D(3) stimulates the expression of several phase I and phase II biotransformation genes. There is also an increased expression of antioxidant genes. These results support the idea that vitamin D is a significant factor in detoxification and protection against environmental toxins.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Biotransformation
  • Calcitriol / pharmacology*
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism
  • Disease Models, Animal
  • Duodenum / drug effects*
  • Duodenum / enzymology*
  • Epoxide Hydrolases / genetics
  • Epoxide Hydrolases / metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Heme Oxygenase (Decyclizing) / genetics
  • Heme Oxygenase (Decyclizing) / metabolism
  • Inactivation, Metabolic / genetics*
  • Male
  • Metabolic Detoxication, Phase I / genetics
  • Metabolic Detoxication, Phase II / genetics
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction
  • Steroid Hydroxylases / genetics
  • Steroid Hydroxylases / metabolism
  • Time Factors
  • Transferases / genetics
  • Transferases / metabolism
  • Vitamin D Deficiency
  • Vitamins / pharmacology*


  • Antioxidants
  • RNA, Messenger
  • Vitamins
  • Cytochrome P-450 Enzyme System
  • Steroid Hydroxylases
  • Heme Oxygenase (Decyclizing)
  • Transferases
  • Epoxide Hydrolases
  • Calcitriol