Abstract
The leukocyte receptor CD62, which is expressed on activated platelets and endothelial cells, is shown to mediate cell adhesion by binding a sialylated carbohydrate structure, sialyl-Lewis x, found on neutrophils, monocytes, and tumor cells. This structure has previously been identified as the ligand for another member of the LEC-CAM family of cell adhesion molecules, endothelial cell-leukocyte adhesion molecule 1, which also binds neutrophils and monocytes. The results demonstrate that although the two LEC-CAMs differ in their biological activities by their distribution and mode of expression, they are capable of mediating cell adhesion by recognition of the same carbohydrate ligand.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Antigens, CD / immunology
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Blood Platelets / immunology
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Blood Platelets / physiology*
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Carbohydrate Conformation
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Carbohydrate Sequence
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Cell Adhesion Molecules / immunology*
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Cell Adhesion*
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Cell Line
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Cells, Cultured
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E-Selectin
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Endothelium, Vascular / immunology
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Endothelium, Vascular / physiology*
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Humans
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Lewis X Antigen / immunology*
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Ligands
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Liposomes
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Molecular Sequence Data
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Neutrophils / immunology
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Neutrophils / physiology*
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P-Selectin
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Platelet Activation
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Platelet Membrane Glycoproteins / immunology*
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Receptors, Immunologic / immunology
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Cell Adhesion Molecules
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E-Selectin
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Lewis X Antigen
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Ligands
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Liposomes
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P-Selectin
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Platelet Membrane Glycoproteins
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Receptors, Immunologic