Immunocytochemical analysis of the cellular infiltrate in primary regressing and non-regressing malignant melanoma

J Invest Dermatol. 1991 Aug;97(2):197-202. doi: 10.1111/1523-1747.ep12479662.


Spontaneous regression occurs in a small proportion of malignant melanomas, and it is important to understand the processes involved in its induction as this may give a guide to future therapies for this disease. We have examined 36 primary malignant melanomas (19 regressing, 17 non-regressing) and identified the cellular phenotypes and activation states of the cells infiltrating regressing and non-regressing primary melanomas by immunochemistry. We have found a significantly increased number of CD3-positive cells and an increased ratio of CD4/CD8-positive cells infiltrating regressing compared to non-regressing tumors. In addition, the expression of the interleukin 2 receptor, an activation marker for T cells, was increased. However, there were no significant differences in class II MHC, CD1, intercellular adhesion molecule 1 (ICAM1), or melanoma-associated differentiation-antigen expression in these tumors. These data are consistent with melanoma regression being induced by activated CD4 T cells and do not seem to be related to the differentiation markers we have examined on these tumors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / metabolism
  • Antibody Formation / immunology
  • Antigen-Presenting Cells / immunology
  • Antigens, Neoplasm / immunology
  • Antigens, Neoplasm / metabolism
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / metabolism
  • Humans
  • Immunohistochemistry
  • L-Selectin
  • Langerhans Cells / immunology
  • Langerhans Cells / metabolism
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Melanoma / immunology
  • Melanoma / metabolism*
  • Melanoma / pathology


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Cell Adhesion Molecules
  • L-Selectin