Oxygen regulated 80 kDa protein and glucose regulated 78kDa protein are identical

Mol Cell Biochem. 1991 May 15;103(2):141-8. doi: 10.1007/BF00227480.


Ischemic stress of cells within solid tumors arises from inadequate perfusion of regions of the tumor and results in microenvironments which are hypoxic and deficient in nutrient delivery and waste product removal. Stressed cells within these microenvironments show growth inhibition and synthesize unique sets of proteins referred to as glucose and oxygen regulated proteins (GRPs and ORPs respectively). The commonality of proteins induced by glucose-starvation and hypoxia has not been proven. To this end, ORPs were induced in Chinese hamster ovary cells in the presence of high glucose concentration in the media and ORP 80 isolated from two dimension gels. Eleven tryptic peptides of the 80 kDa ORP were sequenced and found to be identical to GRP 78 sequences. The data demonstrate that GRP 78 and ORP 80 have the same primary amino acid sequence and suggest that glucose-starvation and hypoxia can induce the same cellular responses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Autoradiography
  • Binding Sites
  • Cell Division
  • Cell Hypoxia / physiology*
  • Cell Line
  • Electrophoresis, Gel, Two-Dimensional
  • Glucose / metabolism*
  • HSP70 Heat-Shock Proteins*
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Protein Binding
  • Proteins / chemistry*
  • Proteins / metabolism
  • Staining and Labeling


  • HSP70 Heat-Shock Proteins
  • Membrane Proteins
  • Proteins
  • glucose-regulated proteins
  • oxygen-regulated proteins
  • Glucose