Novel fluorescent proteins for high-content screening

Drug Discov Today. 2006 Dec;11(23-24):1054-60. doi: 10.1016/j.drudis.2006.09.005. Epub 2006 Sep 25.


The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy to pharmaceutical high-throughput drug-discovery assays, referred to as high-content screening (HCS). Initially, green fluorescent protein and its derivatives from Aequorea Victoria, and later anthozoan fluorescent proteins (FPs) have become potent tools as live-cell markers in HCS assays. We illustrate the broad applicability of classic and novel FPs to drug-discovery assays, giving example applications of the use of FPs in multiplexed imaging as fluorescent timers, photosensitizers and pulse-chase labels, and for robotically integrated compound testing.

Publication types

  • Review

MeSH terms

  • Animals
  • Drug Design
  • Drug Evaluation, Preclinical
  • Luminescent Measurements / methods
  • Luminescent Proteins / chemistry
  • Luminescent Proteins / metabolism*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods
  • Pharmaceutical Preparations / chemistry
  • Pharmaceutical Preparations / metabolism*
  • Technology, Pharmaceutical / methods


  • Luminescent Proteins
  • Pharmaceutical Preparations
  • red fluorescent protein