The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis

Cell. 1991 Jul 26;66(2):233-43. doi: 10.1016/0092-8674(91)90614-5.


Mouse anti-Fas monoclonal antibody has a cytolytic activity on human cells that express the antigen. Complementary DNAs encoding the cell surface antigen Fas were isolated from a cDNA library of human T cell lymphoma KT-3 cells. The nucleotide sequence of the cDNAs revealed that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide (Mr 36,000) with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. Murine WR19L cells or L929 cells transformed with the human Fas antigen cDNA were killed by the anti-Fas antibody in the process known as apoptosis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Surface / genetics
  • Antigens, Surface / physiology*
  • Base Sequence
  • Blotting, Northern
  • Cell Line
  • Cell Survival*
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • Gene Expression
  • Gene Library
  • Humans
  • L Cells / ultrastructure
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Protein Conformation
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transfection
  • fas Receptor


  • Antigens, Surface
  • DNA, Neoplasm
  • RNA, Messenger
  • Recombinant Proteins
  • fas Receptor

Associated data

  • GENBANK/M60962
  • GENBANK/M64383
  • GENBANK/M64384
  • GENBANK/M67454
  • GENBANK/M74093
  • GENBANK/S44028
  • GENBANK/S56134
  • GENBANK/S56136
  • GENBANK/S56137
  • GENBANK/S56139