Formation of Pseudorabies virus glycoprotein E/I complex in baculovirus recombinant system

Acta Virol. 2006;50(3):169-74.

Abstract

Glycoproteins E (gE) and I (gI) of Pseudorabies virus (PRV) form a non-covalently bound complex to which a number of functions have been attributed. The gE/gI complex formation was studied using a series of full-length and truncated forms of gE and gI expressed in baculovirus recombinant system. Both glycoproteins were truncated by stepwise removal of their C-terminal parts and their ability to form the complex was studied by radioimmunoprecipitation. It was found that N-terminal domains of gE and gI containing first 122 and 106 aa, respectively, were sufficient for the complex formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baculoviridae / genetics*
  • Baculoviridae / metabolism
  • Cells, Cultured
  • Herpesvirus 1, Suid / genetics
  • Herpesvirus 1, Suid / metabolism*
  • Radioimmunoprecipitation Assay
  • Recombination, Genetic*
  • Spodoptera / virology
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / metabolism*

Substances

  • Viral Envelope Proteins
  • glycoprotein E, Suid herpesvirus 1
  • pseudorabies virus glycoproteins