Immune enhancing effects of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice

Int J Food Microbiol. 2007 Apr 1;115(1):115-8. doi: 10.1016/j.ijfoodmicro.2006.10.007. Epub 2006 Nov 28.

Abstract

The immune enhancing properties of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice were investigated. Each mouse (BALB/c) was orally fed with cultures of either L. acidophilus or L. paracasei at 10(8) CFU/50 mul per day for 14 days. The effect of these strains on immunoglobulin A, interleukin-10 and interferon gamma producing cells in the gut immune system was determined by immunofluorescence assays. Systemic immune responses were analysed in mice serum upon euthanising after a 14-day feeding trial to estimate cytokines such as IL-10 and IFN-gamma by enzyme-linked immunosorbent assays. L. acidophilus and L. paracasei strains demonstrated an increase in the number of IgA producing cells, IL-10 and IFN-gamma cytokine producing cells in the small intestine. In the systemic immune response, mice fed with L. acidophilus or L. paracasei also enhanced the secretion of anti-inflammatory cytokine (IL-10) and pro-inflammatory cytokine (IFN-gamma). The results of this study suggest that L. acidophilus and L. paracasei were able to enhance specific gut and systemic immune responses in mice.

MeSH terms

  • Animals
  • Colony Count, Microbial
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique, Direct
  • Immunoglobulin A / metabolism*
  • Interferon-gamma / biosynthesis*
  • Interleukin-10 / biosynthesis*
  • Intestine, Small / immunology*
  • Intestine, Small / microbiology*
  • Lactobacillus / immunology*
  • Lactobacillus acidophilus / immunology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Random Allocation

Substances

  • Immunoglobulin A
  • Interleukin-10
  • Interferon-gamma