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. 2007 Feb;45(2):402-8.
doi: 10.1128/JCM.01456-06. Epub 2006 Nov 29.

Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance From Helicobacter Pylori to Campylobacter Jejuni

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Free PMC article

Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance From Helicobacter Pylori to Campylobacter Jejuni

Omar A Oyarzabal et al. J Clin Microbiol. .
Free PMC article

Abstract

Many strains of Helicobacter pylori are naturally competent for transformation and able to transfer chromosomal DNA among different isolates using a conjugation-like mechanism. In this study, we sought to determine whether H. pylori can transfer DNA into Campylobacter jejuni, a closely related species of the Campylobacterales group. To monitor the transfer, a chromosomally encoded streptomycin resistance cassette prearranged by a specific mutation in the rpsL gene of H. pylori was used. Mating of the bacteria on plates or in liquid broth medium produced C. jejuni progeny containing the streptomycin marker. DNA transfer was unidirectional, from H. pylori to C. jejuni, and the progeny were genetically identical to C. jejuni recipient strains. DNase I treatment reduced but did not eliminate transfer, and DNase I-treated cell supernatants did not transform, ruling out phage transduction. Recombinants also did not occur when the mating bacteria were separated by a membrane, suggesting that DNA transfer requires cell-to-cell contact. Transfer of the streptomycin marker was independent of the H. pylori comB transformation system, the cag pathogenicity island, and another type IV secretion system called tfs3. These findings indicated that a DNase I-resistant, conjugation-like mechanism may contribute to horizontal DNA transfer between different members of the Campylobacteriales group. The significance of this DNA uptake by C. jejuni in the context of acquiring antibiotic resistance is discussed.

Figures

FIG. 1.
FIG. 1.
RAPD-PCR analysis of H. pylori and C. jejuni recombinants obtained from mating between the indicated parental strains (A and D) using primer D9355 (B and E). Antibiotic resistance phenotypes of selected Strr/Sptr doubly resistant recombinants are shown. Agar plates contained 10 μg/ml streptomycin, 10 μg/ml spectinomycin, 8 μg/ml kanamycin, or 10 μg/ml rifampin. A mixture of progeny showed profiles identical to that of the C. jejuni parental strain, which is consistent with unidirectional DNA transfer from H. pylori into C. jejuni. (C and F) Control PCRs for the indicated H. pylori and C. jejuni genes.

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