Striatal restricted adenosine A2 receptor (RDC8) is expressed by enkephalin but not by substance P neurons: an in situ hybridization histochemistry study

J Neurochem. 1991 Sep;57(3):1062-7. doi: 10.1111/j.1471-4159.1991.tb08257.x.

Abstract

RDC8 has been recently cloned and characterized as an adenosine A2 receptor. This receptor is expressed exclusively by medium-sized neurons of the striatum as demonstrated by in situ hybridization. We have now studied the relationship of this receptor with three major components of the rat caudate-putamen: enkephalin, substance P, and choline acetyltransferase. Our results demonstrate that the adenosine A2 receptor is expressed exclusively by the enkephalinergic striatal subpopulation but not by the substance P-containing or cholinergic neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Choline O-Acetyltransferase / genetics
  • Choline O-Acetyltransferase / metabolism
  • Choline O-Acetyltransferase / physiology
  • Corpus Striatum / metabolism
  • Corpus Striatum / physiology
  • Corpus Striatum / ultrastructure*
  • Enkephalins / genetics
  • Enkephalins / metabolism*
  • Enkephalins / physiology
  • Gene Expression
  • Histocytochemistry
  • Male
  • Neurons / metabolism*
  • Neurons / physiology
  • Neurons / ultrastructure
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Inbred Strains
  • Receptors, Purinergic / genetics
  • Receptors, Purinergic / metabolism*
  • Receptors, Purinergic / physiology
  • Substance P / genetics
  • Substance P / metabolism*
  • Substance P / physiology

Substances

  • Enkephalins
  • RNA, Messenger
  • Receptors, Purinergic
  • Substance P
  • Choline O-Acetyltransferase