Analysis of the soluble ATP-binding proteome of plant mitochondria identifies new proteins and nucleotide triphosphate interactions within the matrix

Jun Ito; Joshua L Heazlewood; A Harvey Millar

doi:10.1021/pr060403j

Analysis of the soluble ATP-binding proteome of plant mitochondria identifies new proteins and nucleotide triphosphate interactions within the matrix

J Proteome Res. 2006 Dec;5(12):3459-69. doi: 10.1021/pr060403j.

Authors

Jun Ito¹, Joshua L Heazlewood, A Harvey Millar

Affiliation

¹ ARC Centre of Excellence in Plant Energy Biology and School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, M316, Crawley 6009, WA, Australia.

PMID: 17137349
DOI: 10.1021/pr060403j

Abstract

The interactions of ATP inside plant mitochondria were investigated by identifying the soluble nucleotide binding proteome captured using immobilized ATP. Selected proteins were separated by 1D SDS-PAGE and 2D IEF-SDS-PAGE and identified by ESI-Q-TOF MS/MS. A range of highly enriched proteins were identified from the mitochondrial proteome, including 14-3-3 proteins and RNA binding proteins, as well as proteins known to contain nucleotide binding domains and/or to be inhibited or stimulated by ATP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP-Binding Cassette Transporters / analysis
ATP-Binding Cassette Transporters / metabolism*
Adaptor Proteins, Signal Transducing / analysis
Adaptor Proteins, Signal Transducing / metabolism
Arabidopsis / chemistry*
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Mass Spectrometry
Mitochondria / chemistry*
Mitochondrial Proteins / analysis*
Mitochondrial Proteins / metabolism
Plant Proteins / analysis*
Plant Proteins / metabolism
Proteomics*

Substances

ATP-Binding Cassette Transporters
Adaptor Proteins, Signal Transducing
Mitochondrial Proteins
Plant Proteins