Functional and quantitative proteomics using SILAC

Nat Rev Mol Cell Biol. 2006 Dec;7(12):952-8. doi: 10.1038/nrm2067.


Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acids / analysis
  • Amino Acids / metabolism
  • Animals
  • Humans
  • Isotope Labeling / methods*
  • Mass Spectrometry
  • Protein Interaction Mapping
  • Proteins / chemistry*
  • Proteomics / methods*


  • Amino Acids
  • Proteins