Abstract
Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Arabidopsis / genetics*
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Cysteine
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Fluoresceins*
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Fluorescent Dyes*
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Microscopy, Confocal
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Nicotiana / genetics*
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Organometallic Compounds*
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Recombinant Proteins / analysis*
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Recombinant Proteins / metabolism
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Repetitive Sequences, Amino Acid
Substances
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4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein
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Fluoresceins
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Fluorescent Dyes
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Organometallic Compounds
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Recombinant Proteins
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Cysteine