FlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco

Biotechniques. 2006 Nov;41(5):569-70, 572-4. doi: 10.2144/000112264.

Abstract

Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics*
  • Cysteine
  • Fluoresceins*
  • Fluorescent Dyes*
  • Microscopy, Confocal
  • Nicotiana / genetics*
  • Organometallic Compounds*
  • Recombinant Proteins / analysis*
  • Recombinant Proteins / metabolism
  • Repetitive Sequences, Amino Acid

Substances

  • 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein
  • Fluoresceins
  • Fluorescent Dyes
  • Organometallic Compounds
  • Recombinant Proteins
  • Cysteine