Phosphorylation-regulated Cl- channel in CHO cells stably expressing the cystic fibrosis gene

Nature. 1991 Aug 15;352(6336):628-31. doi: 10.1038/352628a0.


A cyclic AMP-stimulated chloride conductance appears when the cystic fibrosis gene is expressed in non-epithelial cells by infection with recombinant viruses. Cyclic AMP-stimulated conductance in this system is mediated by the same ohmic, low-conductance Cl- channel as in human secretory epithelia, but control of this channel by phosphorylation has not been directly demonstrated. Here we report the appearance of the low-conductance Cl- channel in Chinese hamster ovary cells after stable transfection with the cystic fibrosis gene. The channel is regulated on-cell by membrane-permeant analogues of cAMP and off-cell by protein kinases A and C and by alkaline phosphatase. These results are further evidence that the cystic fibrosis transmembrane regulator is a Cl- channel which can be activated by specific phosphorylation events and inactivated by dephosphorylation; they reveal an unsuspected synergism between converging kinase regulatory pathways.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Chloride Channels
  • Cloning, Molecular
  • Cricetinae
  • Cricetulus
  • Cyclic AMP / physiology
  • Cystic Fibrosis / genetics
  • Cystic Fibrosis / physiopathology*
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Humans
  • In Vitro Techniques
  • Ion Channel Gating
  • Membrane Proteins / physiology*
  • Phosphorylation
  • Protein Kinases / physiology
  • Transfection


  • CFTR protein, human
  • Chloride Channels
  • Membrane Proteins
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Cyclic AMP
  • Protein Kinases